Review



p jak1  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Cell Signaling Technology Inc p jak1
    P Jak1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 311 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+jak1/Phospho-Jak1+(Tyr1034%2F1035)+Antibody/pmc13039202-279-32-80
    Average 95 stars, based on 311 article reviews
    p jak1 - by Bioz Stars, 2026-10
    95/100 stars

    Images

    Related Articles

    Bioprocessing:

    Article Title: Lumpy skin disease virus protein LSDV122 impairs IFN-I receptor complex formation to evade host innate immunity.
    Article Snippet: .. Mouse monoclonal antibodies against HA (66006, Proteintech), Rabbit monoclonal antibodies against HA (H6908, Sigma), Mouse monoclonal antibodies against Flag (AP0530, Sigma), HRP-Flag (ZB15939, Servicebio), IgG (I5381 and I5006, Sigma), p-JAK1 (3331S, Cell Signaling Technology), JAK1 (A25841, ABclonal), p-TYK2 and TYK2 (9321S and 14193S, Cell Signaling Technology), p-STAT1 (9167S, Cell Signaling Technology), STAT1 (sc-417, Santa Cruz Biotechnology), p-STAT2 and STAT2 (88410S and 72604S, Cell Signaling Technology), IFNAR1 (A0575, ABclonal), IFNAR2 (53883s, Cell Signaling Technology), PE anti-DYKDDDDK Tag (Biolegend, 637310), Anti-mouse IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 594 Conjugate) (8890, Cell Signaling Technology) and Alexa Fluor 488 goat anti-rabbit IgG (H + L) (A11008, Invitrogen) were purchased from the specified manufacturers. ..

    Article Title: Lumpy skin disease virus protein LSDV122 impairs IFN-I receptor complex formation to evade host innate immunity
    Article Snippet: Trypsin (C100C1, NCM Biotech), fetal bovine serum (SA211.02, Cellmax), penicillin and streptomycin ( PB180120 , Pricella), Dulbecco’s Modified Eagle Medium (C11965500BT, Gibco), puromycin (AMR-J593, VWR), protein A + G agarose (P2055, Beyotime), PMSF (P7626, Sigma), recombinant human IFN-β (300–02BC, ThermoFisher Scientific), recombinant human IFN-γ (300–02, ThermoFisher Scientific), SYBR Green Supermix (Q312, Vazyme), HiScript II Select RT SuperMix for qPCR (R323, Vazyme), and aprotin, leupeptin, β-glycerophosphate disodium salt, Ara-C, CHX and sodium orthovanadate (HY-P0017, HY-18234A, HY-126304, HY-13605, HY-12320 and HY-D0852 respectively, MCE) were purchased from the specified manufacturers. .. Mouse monoclonal antibodies against HA (66006, Proteintech), Rabbit monoclonal antibodies against HA (H6908, Sigma), Mouse monoclonal antibodies against Flag (AP0530, Sigma), HRP-Flag (ZB15939, Servicebio), IgG (I5381 and I5006, Sigma), p-JAK1 (3331S, Cell Signaling Technology), JAK1 (A25841, ABclonal), p-TYK2 and TYK2 (9321S and 14193S, Cell Signaling Technology), p-STAT1 (9167S, Cell Signaling Technology), STAT1 (sc-417, Santa Cruz Biotechnology), p-STAT2 and STAT2 (88410S and 72604S, Cell Signaling Technology), IFNAR1 (A0575, ABclonal), IFNAR2 (53883s, Cell Signaling Technology), PE anti-DYKDDDDK Tag (Biolegend, 637310), Anti-mouse IgG (H + L), F(ab’)2 Fragment (Alexa Fluor 594 Conjugate) (8890, Cell Signaling Technology) and Alexa Fluor 488 goat anti-rabbit IgG (H + L) (A11008, Invitrogen) were purchased from the specified manufacturers. ..

    Incubation:

    Article Title: ShenQi DiHuang Decoction (SQDHD) Ameliorates Neuroinflammation and Neuropsychiatric Manifestations in Pristane Induced Lupus Mice via Blocking JAK1‐STAT3 Pathway
    Article Snippet: .. The membranes were blocked with 5% non‐fat milk for 2 h and incubated overnight at 4°C with the following primary antibodies: VCAM‐1 (1:2000), P‐selectin (1:500), JAK1 (1:1000; Cell Signaling Technology, Boston, USA), p‐JAK1 (1:1000; Cell Signaling Technology), STAT3 (1:2000, Cell Signaling Technology) and p‐STAT3 (1:2000, Cell Signaling Technology). .. Following this, the membranes were incubated with a horseradish peroxidase‐conjugated IgG secondary antibody (1:10000; Proteintech, Wuhan, CHN) at room temperature for 2 h. Protein bands were visualized using an ECL chemiluminescence kit (absin, Shanghai, CHN) and then imaged with a luminescent image analyzer (cytiva, Tokyo, JPN).

    Article Title: Discovery of synthetic G-quadruplex DNA as SARS-CoV-2 helicase inhibitor with antiviral, anti-inflammatory and antioxidative properties
    Article Snippet: .. The membranes were blocked in blocking buffer (TBS [pH 7.6], 0.1% Tween-20, 5% nonfat dry milk) for at least 3 h at 4 °C and then incubated with the following primary antibody: p-JAK1 (Tyr1022/1023, #3331S, 1:1000), JAK1 (#3344 1:1000), p-JAK2 (Tyr1008‚ #8082S, 1:1000), JAK2 (#3230, 1:1000), p-TYK2 (Tyr1054/1055, #68790, 1:1000), TYK2 (#14193, 1:1000), p-STAT1 (Tyr701, #9171, 1:1000), STAT1 (#14994, 1:1000), p-STAT2 (Tyr690‚ #88410, 1:1000), STAT2 (#72604, 1:1000), p-STAT3 (Tyr705, #9145S, 1:500), STAT3 (#9139, 1:1000) and beta-actin (#3700, 1:2000) (all from CST, Frankfurt, Germany). .. HBE ALI lysates were analyzed with primary antibodies against GAPDH (#2118, 1:4000, CST), ISG15 (#sc-166755,1:200, Santa Cruz Biotechnology), Mx1 (#37849, 1:1000, CST), SARS-CoV-2 Nucleocapsid (#40143-R019, 1:10 000, Sino Biological), STAT1 (#9172, 1:1000, CST), phospho-STAT1 Y701 (#9171, 1:1000, CST).

    Article Title: Berberine suppresses hepatocellular carcinoma progression by blocking IL-4-JAK1-STAT6-mediated M2 polarization of macrophage
    Article Snippet: Samples were separated on 8% or 10% acrylamide gels by electrophoresis at 120 V. Proteins were transferred to nitrocellulose membranes (GE Healthcare, Little Chalfont, UK) and blocked with 5% skim milk for 1.5 h to prevent nonspecific binding. .. Primary antibodies were incubated overnight at 4 °C, including: p-STAT6 (56554S, CST), t-STAT6 (9362S, CST), p-JAK1 (3331S, CST), t-JAK1 (3344S, CST), β-actin (8457S, CST), Arg-1 (89872SF, CST), Retnla (Abcam, AB39626). .. Secondary antibodies were incubated for 2 h. After washing, proteins were visualized using ECL detection reagent (ThermoFisher Scientific, Waltham, USA) and a ChemiDoc XRS + system (Bio-Rad, Hercules, USA).

    Article Title: TGM2 Aggravates Acute Pancreatitis by Impairing Macrophage Efferocytosis Through Inhibition of the STAT6-GAS6 Axis.
    Article Snippet: .. The membranes were blocked with 5% skim milk at room temperature for 1 h. Primary antibodies—TGM2 (Proteintech, 15100-1-AP, 1:20000), GAS6 (Proteintech, 13795-1- P, 1:1000), STAT6 (CST, #5397, 1:1000), p-STAT6 (CST, #56554, 1:1000), p-JAK1 (CST, #3331, 1:1000) and p-JAK3 (Proteintech, 29101-1-AP, 1:1000)—were incubated overnight at 4◦C. .. After washing with PBST, fluorescent secondary antibodies (LICOR) were added and incubated at room temperature for 1 h, followed by additional washing.

    Article Title: Targeting CXCL8 alleviates intestinal epithelial inflammation by suppressing the JAK-STAT pathway.
    Article Snippet: The protein samples were separated by SDS-PAGE and moved onto a PVDF film (FFP32, Beyotime, Shanghai, China). .. To prevent non- AR TIC LE IN PR ES S specific binding, the membrane was then blocked with 5% skimmed milk for 2 h and then incubated with primary antibodies including p-JAK1 (1:1000, 3331, Cell Signaling Technology (CST), Danvers, MA) (https://www.cellsignal.com/), JAK1 (1:1000, 3332, CST), p-JAK2 (1:1000, 3771, CST), JAK2 (1:1000, 3230, CST), p-STAT1 (1:1000, 9167, CST), STAT1 (1:1000, 9172, CST), p-STAT3 (1:1000, 9131, CST), STAT3 (1:2000, 4904, CST) and internal reference β-actin (1:1000, 4967, CST) overnight at 4°C. .. Goat anti-rabbit IgG H&L (HRP) (1:2500 dilution, ab205718, Abcam, Cambridge, UK) was further incubated with the film for 1.5 h at room temperature.

    Blocking Assay:

    Article Title: Discovery of synthetic G-quadruplex DNA as SARS-CoV-2 helicase inhibitor with antiviral, anti-inflammatory and antioxidative properties
    Article Snippet: .. The membranes were blocked in blocking buffer (TBS [pH 7.6], 0.1% Tween-20, 5% nonfat dry milk) for at least 3 h at 4 °C and then incubated with the following primary antibody: p-JAK1 (Tyr1022/1023, #3331S, 1:1000), JAK1 (#3344 1:1000), p-JAK2 (Tyr1008‚ #8082S, 1:1000), JAK2 (#3230, 1:1000), p-TYK2 (Tyr1054/1055, #68790, 1:1000), TYK2 (#14193, 1:1000), p-STAT1 (Tyr701, #9171, 1:1000), STAT1 (#14994, 1:1000), p-STAT2 (Tyr690‚ #88410, 1:1000), STAT2 (#72604, 1:1000), p-STAT3 (Tyr705, #9145S, 1:500), STAT3 (#9139, 1:1000) and beta-actin (#3700, 1:2000) (all from CST, Frankfurt, Germany). .. HBE ALI lysates were analyzed with primary antibodies against GAPDH (#2118, 1:4000, CST), ISG15 (#sc-166755,1:200, Santa Cruz Biotechnology), Mx1 (#37849, 1:1000, CST), SARS-CoV-2 Nucleocapsid (#40143-R019, 1:10 000, Sino Biological), STAT1 (#9172, 1:1000, CST), phospho-STAT1 Y701 (#9171, 1:1000, CST).

    Binding Assay:

    Article Title: Targeting CXCL8 alleviates intestinal epithelial inflammation by suppressing the JAK-STAT pathway.
    Article Snippet: The protein samples were separated by SDS-PAGE and moved onto a PVDF film (FFP32, Beyotime, Shanghai, China). .. To prevent non- AR TIC LE IN PR ES S specific binding, the membrane was then blocked with 5% skimmed milk for 2 h and then incubated with primary antibodies including p-JAK1 (1:1000, 3331, Cell Signaling Technology (CST), Danvers, MA) (https://www.cellsignal.com/), JAK1 (1:1000, 3332, CST), p-JAK2 (1:1000, 3771, CST), JAK2 (1:1000, 3230, CST), p-STAT1 (1:1000, 9167, CST), STAT1 (1:1000, 9172, CST), p-STAT3 (1:1000, 9131, CST), STAT3 (1:2000, 4904, CST) and internal reference β-actin (1:1000, 4967, CST) overnight at 4°C. .. Goat anti-rabbit IgG H&L (HRP) (1:2500 dilution, ab205718, Abcam, Cambridge, UK) was further incubated with the film for 1.5 h at room temperature.

    Membrane:

    Article Title: Targeting CXCL8 alleviates intestinal epithelial inflammation by suppressing the JAK-STAT pathway.
    Article Snippet: The protein samples were separated by SDS-PAGE and moved onto a PVDF film (FFP32, Beyotime, Shanghai, China). .. To prevent non- AR TIC LE IN PR ES S specific binding, the membrane was then blocked with 5% skimmed milk for 2 h and then incubated with primary antibodies including p-JAK1 (1:1000, 3331, Cell Signaling Technology (CST), Danvers, MA) (https://www.cellsignal.com/), JAK1 (1:1000, 3332, CST), p-JAK2 (1:1000, 3771, CST), JAK2 (1:1000, 3230, CST), p-STAT1 (1:1000, 9167, CST), STAT1 (1:1000, 9172, CST), p-STAT3 (1:1000, 9131, CST), STAT3 (1:2000, 4904, CST) and internal reference β-actin (1:1000, 4967, CST) overnight at 4°C. .. Goat anti-rabbit IgG H&L (HRP) (1:2500 dilution, ab205718, Abcam, Cambridge, UK) was further incubated with the film for 1.5 h at room temperature.



    Similar Products

    94
    MedChemExpress phospho jak p jak
    <t>EFHD2</t> influences lung cancer cell proliferation, apoptosis, migration, invasion, and various signaling pathways. (a) WB analysis depicting EFHD2 protein expression in normal bronchial epithelial cells (BEAS-2B) and different lung cancer cell lines (A549, NCI-H1299, and HCC827). (b) Relative mRNA levels of EFHD2 in BEAS-2B and lung cancer cell lines, quantified through qRT-PCR. The data are presented as mean ± SD. (c) WB illustrating EFHD2 protein expression levels. (d) qRT-PCR analysis of EFHD2 mRNA expression. (e) CCK-8 assay assessing the impact of EFHD2 OE and KD on cell proliferation; data were presented as OD450 values over 72 h. (f) Flow cytometry analysis evaluating apoptosis rates. (g) Transwell migration and invasion assays. (h) Colony formation assay. (i) WB analysis of <t>JAK</t> and STAT protein expression, using GAPDH as a loading control. Data are expressed as mean ± SD. ✶ P < 0.05, ✶ ✶ P < 0.01, ✶ ✶ ✶ P < 0.001 compared with the A549 group; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with the NCI-H1299 group. The scale bar represents 100 µm. EFHD2: EF-hand domain-containing protein 2, WB: Western blot, JAK: Janus Kinase, STAT: Signal transducers and activators of transcription, GAPDH: Glyceraldehyde 3-phosphate dehydrogenase, qRT-PCR: Quantitative real-time polymerase chain reaction, CCK-8: Cell counting kit 8, OE: Overexpression, KD: Knockdown, mRNA: Messenger RNA, OD450: Optical density at 450 nm, SD: Standard deviation.
    Phospho Jak P Jak, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+jak1/JAK1+Antibody/pmc13098384-88-24-22
    Average 94 stars, based on 1 article reviews
    phospho jak p jak - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    94
    Bioss p jak1
    <t>EFHD2</t> influences lung cancer cell proliferation, apoptosis, migration, invasion, and various signaling pathways. (a) WB analysis depicting EFHD2 protein expression in normal bronchial epithelial cells (BEAS-2B) and different lung cancer cell lines (A549, NCI-H1299, and HCC827). (b) Relative mRNA levels of EFHD2 in BEAS-2B and lung cancer cell lines, quantified through qRT-PCR. The data are presented as mean ± SD. (c) WB illustrating EFHD2 protein expression levels. (d) qRT-PCR analysis of EFHD2 mRNA expression. (e) CCK-8 assay assessing the impact of EFHD2 OE and KD on cell proliferation; data were presented as OD450 values over 72 h. (f) Flow cytometry analysis evaluating apoptosis rates. (g) Transwell migration and invasion assays. (h) Colony formation assay. (i) WB analysis of <t>JAK</t> and STAT protein expression, using GAPDH as a loading control. Data are expressed as mean ± SD. ✶ P < 0.05, ✶ ✶ P < 0.01, ✶ ✶ ✶ P < 0.001 compared with the A549 group; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with the NCI-H1299 group. The scale bar represents 100 µm. EFHD2: EF-hand domain-containing protein 2, WB: Western blot, JAK: Janus Kinase, STAT: Signal transducers and activators of transcription, GAPDH: Glyceraldehyde 3-phosphate dehydrogenase, qRT-PCR: Quantitative real-time polymerase chain reaction, CCK-8: Cell counting kit 8, OE: Overexpression, KD: Knockdown, mRNA: Messenger RNA, OD450: Optical density at 450 nm, SD: Standard deviation.
    P Jak1, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+jak1/Jak1+(Tyr1034+%2B+Tyr1035)+Antibody/pmc12991359-203-83-85
    Average 94 stars, based on 1 article reviews
    p jak1 - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    86
    Servicebio Inc p jak1 protein antibody
    <t>EFHD2</t> influences lung cancer cell proliferation, apoptosis, migration, invasion, and various signaling pathways. (a) WB analysis depicting EFHD2 protein expression in normal bronchial epithelial cells (BEAS-2B) and different lung cancer cell lines (A549, NCI-H1299, and HCC827). (b) Relative mRNA levels of EFHD2 in BEAS-2B and lung cancer cell lines, quantified through qRT-PCR. The data are presented as mean ± SD. (c) WB illustrating EFHD2 protein expression levels. (d) qRT-PCR analysis of EFHD2 mRNA expression. (e) CCK-8 assay assessing the impact of EFHD2 OE and KD on cell proliferation; data were presented as OD450 values over 72 h. (f) Flow cytometry analysis evaluating apoptosis rates. (g) Transwell migration and invasion assays. (h) Colony formation assay. (i) WB analysis of <t>JAK</t> and STAT protein expression, using GAPDH as a loading control. Data are expressed as mean ± SD. ✶ P < 0.05, ✶ ✶ P < 0.01, ✶ ✶ ✶ P < 0.001 compared with the A549 group; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with the NCI-H1299 group. The scale bar represents 100 µm. EFHD2: EF-hand domain-containing protein 2, WB: Western blot, JAK: Janus Kinase, STAT: Signal transducers and activators of transcription, GAPDH: Glyceraldehyde 3-phosphate dehydrogenase, qRT-PCR: Quantitative real-time polymerase chain reaction, CCK-8: Cell counting kit 8, OE: Overexpression, KD: Knockdown, mRNA: Messenger RNA, OD450: Optical density at 450 nm, SD: Standard deviation.
    P Jak1 Protein Antibody, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+jak1/a10+antibody+cv+monoclonal+protein+vp1/pmc13043380-47-5-8
    Average 86 stars, based on 1 article reviews
    p jak1 protein antibody - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    95
    Cell Signaling Technology Inc p jak1
    <t>EFHD2</t> influences lung cancer cell proliferation, apoptosis, migration, invasion, and various signaling pathways. (a) WB analysis depicting EFHD2 protein expression in normal bronchial epithelial cells (BEAS-2B) and different lung cancer cell lines (A549, NCI-H1299, and HCC827). (b) Relative mRNA levels of EFHD2 in BEAS-2B and lung cancer cell lines, quantified through qRT-PCR. The data are presented as mean ± SD. (c) WB illustrating EFHD2 protein expression levels. (d) qRT-PCR analysis of EFHD2 mRNA expression. (e) CCK-8 assay assessing the impact of EFHD2 OE and KD on cell proliferation; data were presented as OD450 values over 72 h. (f) Flow cytometry analysis evaluating apoptosis rates. (g) Transwell migration and invasion assays. (h) Colony formation assay. (i) WB analysis of <t>JAK</t> and STAT protein expression, using GAPDH as a loading control. Data are expressed as mean ± SD. ✶ P < 0.05, ✶ ✶ P < 0.01, ✶ ✶ ✶ P < 0.001 compared with the A549 group; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with the NCI-H1299 group. The scale bar represents 100 µm. EFHD2: EF-hand domain-containing protein 2, WB: Western blot, JAK: Janus Kinase, STAT: Signal transducers and activators of transcription, GAPDH: Glyceraldehyde 3-phosphate dehydrogenase, qRT-PCR: Quantitative real-time polymerase chain reaction, CCK-8: Cell counting kit 8, OE: Overexpression, KD: Knockdown, mRNA: Messenger RNA, OD450: Optical density at 450 nm, SD: Standard deviation.
    P Jak1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+jak1/Phospho-Jak1+(Tyr1034%2F1035)+Antibody/pmc13039202-279-32-80
    Average 95 stars, based on 1 article reviews
    p jak1 - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    96
    Cell Signaling Technology Inc experiment p stat3
    <t>EFHD2</t> influences lung cancer cell proliferation, apoptosis, migration, invasion, and various signaling pathways. (a) WB analysis depicting EFHD2 protein expression in normal bronchial epithelial cells (BEAS-2B) and different lung cancer cell lines (A549, NCI-H1299, and HCC827). (b) Relative mRNA levels of EFHD2 in BEAS-2B and lung cancer cell lines, quantified through qRT-PCR. The data are presented as mean ± SD. (c) WB illustrating EFHD2 protein expression levels. (d) qRT-PCR analysis of EFHD2 mRNA expression. (e) CCK-8 assay assessing the impact of EFHD2 OE and KD on cell proliferation; data were presented as OD450 values over 72 h. (f) Flow cytometry analysis evaluating apoptosis rates. (g) Transwell migration and invasion assays. (h) Colony formation assay. (i) WB analysis of <t>JAK</t> and STAT protein expression, using GAPDH as a loading control. Data are expressed as mean ± SD. ✶ P < 0.05, ✶ ✶ P < 0.01, ✶ ✶ ✶ P < 0.001 compared with the A549 group; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with the NCI-H1299 group. The scale bar represents 100 µm. EFHD2: EF-hand domain-containing protein 2, WB: Western blot, JAK: Janus Kinase, STAT: Signal transducers and activators of transcription, GAPDH: Glyceraldehyde 3-phosphate dehydrogenase, qRT-PCR: Quantitative real-time polymerase chain reaction, CCK-8: Cell counting kit 8, OE: Overexpression, KD: Knockdown, mRNA: Messenger RNA, OD450: Optical density at 450 nm, SD: Standard deviation.
    Experiment P Stat3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+jak1/Jak1+Antibody/pmc13008898-67-13-15
    Average 96 stars, based on 1 article reviews
    experiment p stat3 - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    96
    Proteintech p jak1
    <t>EFHD2</t> influences lung cancer cell proliferation, apoptosis, migration, invasion, and various signaling pathways. (a) WB analysis depicting EFHD2 protein expression in normal bronchial epithelial cells (BEAS-2B) and different lung cancer cell lines (A549, NCI-H1299, and HCC827). (b) Relative mRNA levels of EFHD2 in BEAS-2B and lung cancer cell lines, quantified through qRT-PCR. The data are presented as mean ± SD. (c) WB illustrating EFHD2 protein expression levels. (d) qRT-PCR analysis of EFHD2 mRNA expression. (e) CCK-8 assay assessing the impact of EFHD2 OE and KD on cell proliferation; data were presented as OD450 values over 72 h. (f) Flow cytometry analysis evaluating apoptosis rates. (g) Transwell migration and invasion assays. (h) Colony formation assay. (i) WB analysis of <t>JAK</t> and STAT protein expression, using GAPDH as a loading control. Data are expressed as mean ± SD. ✶ P < 0.05, ✶ ✶ P < 0.01, ✶ ✶ ✶ P < 0.001 compared with the A549 group; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with the NCI-H1299 group. The scale bar represents 100 µm. EFHD2: EF-hand domain-containing protein 2, WB: Western blot, JAK: Janus Kinase, STAT: Signal transducers and activators of transcription, GAPDH: Glyceraldehyde 3-phosphate dehydrogenase, qRT-PCR: Quantitative real-time polymerase chain reaction, CCK-8: Cell counting kit 8, OE: Overexpression, KD: Knockdown, mRNA: Messenger RNA, OD450: Optical density at 450 nm, SD: Standard deviation.
    P Jak1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+jak1/JAK1+Antibody/10__1016_slash_j__fbp__2026__03__030-53-61-70
    Average 96 stars, based on 1 article reviews
    p jak1 - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc anti p jak1
    <t>EFHD2</t> influences lung cancer cell proliferation, apoptosis, migration, invasion, and various signaling pathways. (a) WB analysis depicting EFHD2 protein expression in normal bronchial epithelial cells (BEAS-2B) and different lung cancer cell lines (A549, NCI-H1299, and HCC827). (b) Relative mRNA levels of EFHD2 in BEAS-2B and lung cancer cell lines, quantified through qRT-PCR. The data are presented as mean ± SD. (c) WB illustrating EFHD2 protein expression levels. (d) qRT-PCR analysis of EFHD2 mRNA expression. (e) CCK-8 assay assessing the impact of EFHD2 OE and KD on cell proliferation; data were presented as OD450 values over 72 h. (f) Flow cytometry analysis evaluating apoptosis rates. (g) Transwell migration and invasion assays. (h) Colony formation assay. (i) WB analysis of <t>JAK</t> and STAT protein expression, using GAPDH as a loading control. Data are expressed as mean ± SD. ✶ P < 0.05, ✶ ✶ P < 0.01, ✶ ✶ ✶ P < 0.001 compared with the A549 group; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with the NCI-H1299 group. The scale bar represents 100 µm. EFHD2: EF-hand domain-containing protein 2, WB: Western blot, JAK: Janus Kinase, STAT: Signal transducers and activators of transcription, GAPDH: Glyceraldehyde 3-phosphate dehydrogenase, qRT-PCR: Quantitative real-time polymerase chain reaction, CCK-8: Cell counting kit 8, OE: Overexpression, KD: Knockdown, mRNA: Messenger RNA, OD450: Optical density at 450 nm, SD: Standard deviation.
    Anti P Jak1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+jak1/pmc12912577-51-15-18
    Average 86 stars, based on 1 article reviews
    anti p jak1 - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    Image Search Results


    EFHD2 influences lung cancer cell proliferation, apoptosis, migration, invasion, and various signaling pathways. (a) WB analysis depicting EFHD2 protein expression in normal bronchial epithelial cells (BEAS-2B) and different lung cancer cell lines (A549, NCI-H1299, and HCC827). (b) Relative mRNA levels of EFHD2 in BEAS-2B and lung cancer cell lines, quantified through qRT-PCR. The data are presented as mean ± SD. (c) WB illustrating EFHD2 protein expression levels. (d) qRT-PCR analysis of EFHD2 mRNA expression. (e) CCK-8 assay assessing the impact of EFHD2 OE and KD on cell proliferation; data were presented as OD450 values over 72 h. (f) Flow cytometry analysis evaluating apoptosis rates. (g) Transwell migration and invasion assays. (h) Colony formation assay. (i) WB analysis of JAK and STAT protein expression, using GAPDH as a loading control. Data are expressed as mean ± SD. ✶ P < 0.05, ✶ ✶ P < 0.01, ✶ ✶ ✶ P < 0.001 compared with the A549 group; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with the NCI-H1299 group. The scale bar represents 100 µm. EFHD2: EF-hand domain-containing protein 2, WB: Western blot, JAK: Janus Kinase, STAT: Signal transducers and activators of transcription, GAPDH: Glyceraldehyde 3-phosphate dehydrogenase, qRT-PCR: Quantitative real-time polymerase chain reaction, CCK-8: Cell counting kit 8, OE: Overexpression, KD: Knockdown, mRNA: Messenger RNA, OD450: Optical density at 450 nm, SD: Standard deviation.

    Journal: CytoJournal

    Article Title: Mapping the function of EF-hand domain-containing protein 2 and determining its clinical relevance in non-small-cell lung cancer through single-cell transcriptomics

    doi: 10.25259/Cytojournal_29_2025

    Figure Lengend Snippet: EFHD2 influences lung cancer cell proliferation, apoptosis, migration, invasion, and various signaling pathways. (a) WB analysis depicting EFHD2 protein expression in normal bronchial epithelial cells (BEAS-2B) and different lung cancer cell lines (A549, NCI-H1299, and HCC827). (b) Relative mRNA levels of EFHD2 in BEAS-2B and lung cancer cell lines, quantified through qRT-PCR. The data are presented as mean ± SD. (c) WB illustrating EFHD2 protein expression levels. (d) qRT-PCR analysis of EFHD2 mRNA expression. (e) CCK-8 assay assessing the impact of EFHD2 OE and KD on cell proliferation; data were presented as OD450 values over 72 h. (f) Flow cytometry analysis evaluating apoptosis rates. (g) Transwell migration and invasion assays. (h) Colony formation assay. (i) WB analysis of JAK and STAT protein expression, using GAPDH as a loading control. Data are expressed as mean ± SD. ✶ P < 0.05, ✶ ✶ P < 0.01, ✶ ✶ ✶ P < 0.001 compared with the A549 group; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with the NCI-H1299 group. The scale bar represents 100 µm. EFHD2: EF-hand domain-containing protein 2, WB: Western blot, JAK: Janus Kinase, STAT: Signal transducers and activators of transcription, GAPDH: Glyceraldehyde 3-phosphate dehydrogenase, qRT-PCR: Quantitative real-time polymerase chain reaction, CCK-8: Cell counting kit 8, OE: Overexpression, KD: Knockdown, mRNA: Messenger RNA, OD450: Optical density at 450 nm, SD: Standard deviation.

    Article Snippet: The membranes were then exposed overnight at 4°C to primary antibodies targeting EFHD2 (1:1000, ab24368, Abcam, China), JAK1 (1:1000, HY- P80196 , MedChemExpress, China), phospho-JAK (p-JAK) (1:1000, ab138005, Abcam, China), signal transducers and activators of transcription 3 (STAT3) (1:1000, ab68153, Abcam, China), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:1000, ab8245, Abcam, China) (loading control).

    Techniques: Migration, Protein-Protein interactions, Expressing, Quantitative RT-PCR, CCK-8 Assay, Flow Cytometry, Colony Assay, Control, Western Blot, Real-time Polymerase Chain Reaction, Cell Counting, Over Expression, Knockdown, Standard Deviation

    EFHD2 regulates cell proliferation, migration, invasion, apoptosis, and JAK/STAT signaling. (a) Co-IP identified that FLAG-EFHD2 interacted with Myc-JAK1 in A549+EFHD2 OE and NCI-H1299+EFHD2 KD cells. (b) CCK-8 detected cell proliferation. Inhibition of JAK decreased the proliferation of A549+EFHD2 OE cells, while activation of JAK increased the proliferation of NCI-H1299+EFHD2 KD cells. (c) Flow cytometry to detect apoptosis. Inhibition of JAK increased the apoptosis of A549+EFHD2 OE cells, whereas JAK agonist enhanced the apoptosis of NCI-H1299+EFHD2 KD cells. (d) Transwell migration and invasion. Inhibition of JAK reduced the migration and invasion of A549+EFHD2 OE cells, whereas activation of JAK promoted the migration and invasion of NCI-H1299+EFHD2 KD cells. (e) Colony formation assay. Inhibition of JAK decreased the colony formation of A549+EFHD2 OE cells, whereas activation of JAK enhanced the colony formation of NCI-H1299+EFHD2 KD cells. (f) Western blotting. JAK inhibition decreased the levels of p-JAK1 and p-STAT3 in A549+EFHD2 OE cells, whereas JAK activation increased the levels of p-JAK1 and p-STAT3 in NCI-H1299+EFHD2 KD cells. Data are expressed as mean ± SD. ✶ P < 0.05, ✶ ✶ P < 0.01, ✶ ✶ ✶ P < 0.001 compared with the A549 group; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with the NCI-H1299 group. The scale bar represents 100 µm. JAK: Janus Kinase, STAT: Signal transducers and activators of transcription, Co-IP: Co-immunoprecipitation, EFHD2: EF-hand domain-containing protein 2, OE: Overexpression, KD: Knockdown, CCK-8: Cell counting kit 8, p-JAK: Phospho Janus Kinase, p-STAT: Phospho signal transducers and activators of transcription, SD: Standard deviation.

    Journal: CytoJournal

    Article Title: Mapping the function of EF-hand domain-containing protein 2 and determining its clinical relevance in non-small-cell lung cancer through single-cell transcriptomics

    doi: 10.25259/Cytojournal_29_2025

    Figure Lengend Snippet: EFHD2 regulates cell proliferation, migration, invasion, apoptosis, and JAK/STAT signaling. (a) Co-IP identified that FLAG-EFHD2 interacted with Myc-JAK1 in A549+EFHD2 OE and NCI-H1299+EFHD2 KD cells. (b) CCK-8 detected cell proliferation. Inhibition of JAK decreased the proliferation of A549+EFHD2 OE cells, while activation of JAK increased the proliferation of NCI-H1299+EFHD2 KD cells. (c) Flow cytometry to detect apoptosis. Inhibition of JAK increased the apoptosis of A549+EFHD2 OE cells, whereas JAK agonist enhanced the apoptosis of NCI-H1299+EFHD2 KD cells. (d) Transwell migration and invasion. Inhibition of JAK reduced the migration and invasion of A549+EFHD2 OE cells, whereas activation of JAK promoted the migration and invasion of NCI-H1299+EFHD2 KD cells. (e) Colony formation assay. Inhibition of JAK decreased the colony formation of A549+EFHD2 OE cells, whereas activation of JAK enhanced the colony formation of NCI-H1299+EFHD2 KD cells. (f) Western blotting. JAK inhibition decreased the levels of p-JAK1 and p-STAT3 in A549+EFHD2 OE cells, whereas JAK activation increased the levels of p-JAK1 and p-STAT3 in NCI-H1299+EFHD2 KD cells. Data are expressed as mean ± SD. ✶ P < 0.05, ✶ ✶ P < 0.01, ✶ ✶ ✶ P < 0.001 compared with the A549 group; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with the NCI-H1299 group. The scale bar represents 100 µm. JAK: Janus Kinase, STAT: Signal transducers and activators of transcription, Co-IP: Co-immunoprecipitation, EFHD2: EF-hand domain-containing protein 2, OE: Overexpression, KD: Knockdown, CCK-8: Cell counting kit 8, p-JAK: Phospho Janus Kinase, p-STAT: Phospho signal transducers and activators of transcription, SD: Standard deviation.

    Article Snippet: The membranes were then exposed overnight at 4°C to primary antibodies targeting EFHD2 (1:1000, ab24368, Abcam, China), JAK1 (1:1000, HY- P80196 , MedChemExpress, China), phospho-JAK (p-JAK) (1:1000, ab138005, Abcam, China), signal transducers and activators of transcription 3 (STAT3) (1:1000, ab68153, Abcam, China), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:1000, ab8245, Abcam, China) (loading control).

    Techniques: Migration, Co-Immunoprecipitation Assay, CCK-8 Assay, Inhibition, Activation Assay, Flow Cytometry, Colony Assay, Western Blot, Immunoprecipitation, Over Expression, Knockdown, Cell Counting, Standard Deviation