p jak1 (Cell Signaling Technology Inc)
Structured Review
P Jak1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 311 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+jak1/Phospho-Jak1+(Tyr1034%2F1035)+Antibody/pmc13039202-279-32-80
Average 95 stars, based on 311 article reviews
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Bioprocessing:Article Title: Lumpy skin disease virus protein LSDV122 impairs IFN-I receptor complex formation to evade host innate immunity. Article Snippet: .. Mouse monoclonal antibodies against HA (66006, Proteintech), Rabbit monoclonal antibodies against HA (H6908, Sigma), Mouse monoclonal antibodies against Flag (AP0530, Sigma), HRP-Flag (ZB15939, Servicebio), IgG (I5381 and I5006, Sigma), Article Title: Lumpy skin disease virus protein LSDV122 impairs IFN-I receptor complex formation to evade host innate immunity Article Snippet: Trypsin (C100C1, NCM Biotech), fetal bovine serum (SA211.02, Cellmax), penicillin and streptomycin ( PB180120 , Pricella), Dulbecco’s Modified Eagle Medium (C11965500BT, Gibco), puromycin (AMR-J593, VWR), protein A + G agarose (P2055, Beyotime), PMSF (P7626, Sigma), recombinant human IFN-β (300–02BC, ThermoFisher Scientific), recombinant human IFN-γ (300–02, ThermoFisher Scientific), SYBR Green Supermix (Q312, Vazyme), HiScript II Select RT SuperMix for qPCR (R323, Vazyme), and aprotin, leupeptin, β-glycerophosphate disodium salt, Ara-C, CHX and sodium orthovanadate (HY-P0017, HY-18234A, HY-126304, HY-13605, HY-12320 and HY-D0852 respectively, MCE) were purchased from the specified manufacturers. .. Mouse monoclonal antibodies against HA (66006, Proteintech), Rabbit monoclonal antibodies against HA (H6908, Sigma), Mouse monoclonal antibodies against Flag (AP0530, Sigma), HRP-Flag (ZB15939, Servicebio), IgG (I5381 and I5006, Sigma), Incubation:Article Title: ShenQi DiHuang Decoction (SQDHD) Ameliorates Neuroinflammation and Neuropsychiatric Manifestations in Pristane Induced Lupus Mice via Blocking JAK1‐STAT3 Pathway Article Snippet: .. The membranes were blocked with 5% non‐fat milk for 2 h and incubated overnight at 4°C with the following primary antibodies: VCAM‐1 (1:2000), P‐selectin (1:500), JAK1 (1:1000; Cell Signaling Technology, Boston, USA), Article Title: Discovery of synthetic G-quadruplex DNA as SARS-CoV-2 helicase inhibitor with antiviral, anti-inflammatory and antioxidative properties Article Snippet: .. The membranes were blocked in blocking buffer (TBS [pH 7.6], 0.1% Tween-20, 5% nonfat dry milk) for at least 3 h at 4 °C and then incubated with the following primary antibody: Article Title: Berberine suppresses hepatocellular carcinoma progression by blocking IL-4-JAK1-STAT6-mediated M2 polarization of macrophage Article Snippet: Samples were separated on 8% or 10% acrylamide gels by electrophoresis at 120 V. Proteins were transferred to nitrocellulose membranes (GE Healthcare, Little Chalfont, UK) and blocked with 5% skim milk for 1.5 h to prevent nonspecific binding. .. Primary antibodies were incubated overnight at 4 °C, including: p-STAT6 (56554S, CST), t-STAT6 (9362S, CST), Article Title: TGM2 Aggravates Acute Pancreatitis by Impairing Macrophage Efferocytosis Through Inhibition of the STAT6-GAS6 Axis. Article Snippet: .. The membranes were blocked with 5% skim milk at room temperature for 1 h. Primary antibodies—TGM2 (Proteintech, 15100-1-AP, 1:20000), GAS6 (Proteintech, 13795-1- P, 1:1000), STAT6 (CST, #5397, 1:1000), p-STAT6 (CST, #56554, 1:1000), Article Title: Targeting CXCL8 alleviates intestinal epithelial inflammation by suppressing the JAK-STAT pathway. Article Snippet: The protein samples were separated by SDS-PAGE and moved onto a PVDF film (FFP32, Beyotime, Shanghai, China). .. To prevent non- AR TIC LE IN PR ES S specific binding, the membrane was then blocked with 5% skimmed milk for 2 h and then incubated with primary antibodies including Blocking Assay:Article Title: Discovery of synthetic G-quadruplex DNA as SARS-CoV-2 helicase inhibitor with antiviral, anti-inflammatory and antioxidative properties Article Snippet: .. The membranes were blocked in blocking buffer (TBS [pH 7.6], 0.1% Tween-20, 5% nonfat dry milk) for at least 3 h at 4 °C and then incubated with the following primary antibody: Binding Assay:Article Title: Targeting CXCL8 alleviates intestinal epithelial inflammation by suppressing the JAK-STAT pathway. Article Snippet: The protein samples were separated by SDS-PAGE and moved onto a PVDF film (FFP32, Beyotime, Shanghai, China). .. To prevent non- AR TIC LE IN PR ES S specific binding, the membrane was then blocked with 5% skimmed milk for 2 h and then incubated with primary antibodies including Membrane:Article Title: Targeting CXCL8 alleviates intestinal epithelial inflammation by suppressing the JAK-STAT pathway. Article Snippet: The protein samples were separated by SDS-PAGE and moved onto a PVDF film (FFP32, Beyotime, Shanghai, China). .. To prevent non- AR TIC LE IN PR ES S specific binding, the membrane was then blocked with 5% skimmed milk for 2 h and then incubated with primary antibodies including |
